| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sperry, Zachary J (12)
|
NITTSC |
28.84 |
29.25 |
0.41 |
| 2 |
Wissel, Paul J (11)
|
NITTSC |
29.33 |
29.26 |
-0.07 |
| 3 |
Webster, Hayne W (12)
|
NIUN |
32.00 |
30.44 |
-1.56 |
| 4 |
Bruzgul, Jaxon R (11)
|
NITWST |
32.25 |
30.56 |
-1.69 |
| 5 |
Trometer, Joshua H (12)
|
NIEAST |
33.02 |
32.84 |
-0.18 |
| 6 |
Knowles, Brody C (12)
|
NIPVSC |
32.31 |
33.54 |
1.23 |
| 7 |
Kirchberger, Luke A (11)
|
NIHAMB |
35.95 |
35.25 |
-0.70 |
| 8 |
Krajewski, Benjamin R (12)
|
NIGWST |
37.61 |
36.53 |
-1.08 |
| 9 |
Kopp, Daenon M (11)
|
NITTSC |
36.27 |
36.58 |
0.31 |
| 10 |
Merrill, Christian T (11)
|
NIGWST |
35.59 |
36.69 |
1.10 |
| 11 |
Rignel, Dean P (12)
|
NIEAST |
35.26 |
36.79 |
1.53 |
| 12 |
Kaus, Hayden G (11)
|
NIGWST |
38.96 |
36.98 |
-1.98 |
| 13 |
Bartolotti, Harry D (12)
|
NITWST |
40.35 |
39.50 |
-0.85 |
| 14 |
Andrew, Jacob D (11)
|
NIHAMB |
40.42 |
40.86 |
0.44 |
| 15 |
Harper, Flynn T (12)
|
NIHAMB |
41.01 |
42.88 |
1.87 |
| 16 |
Rugg, Matthew T (12)
|
NIPVSC |
43.32 |
43.05 |
-0.27 |
| 17 |
Molina Ayquipa, Santiago I (11)
|
NITWST |
42.79 |
43.81 |
1.02 |
| 18 |
Barlow, Andrew D (12)
|
NIGWST |
44.00 |
44.27 |
0.27 |
| 19 |
Lindquist, Benjamin M (11)
|
NIPVSC |
45.76 |
45.08 |
-0.68 |
| 20 |
Wyles, Cade B (11)
|
NITTSC |
|
45.34 |
- |
| 21 |
Lorenz, Robert D (12)
|
NITTSC |
50.75 |
50.72 |
-0.03 |
|
Egloff, Ethan B (12)
|
NIEAST |
30.97 |
NS |
- |